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Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
Entomopathogenic fungi, 
and 
, are important agents to control insect pests, but the fungi are highly susceptible to conventional fungicides for plant diseases. The purpose of this study is to generate mutants tolerant to fungicides (benomyl and triflumizole) using quantum beams (
rays and carbon ion beams). Eighteen fungicide-tolerant mutants were selected on the medium supplemented by fungicides (1,000 ppm for benomyl and 150 ppm for trifulmizole). In 5 mutants, fungicide tolerant level, crossing relation with other fungicide and pathogenicity against host insects were investigated. As a result, the mutants that have the minimum inhibitory concentration for benomyl of approximately 5 times were successfully obtained, and yet these were maintained the pathogenicity.
Fitriana, Y.*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
Thermotolerant mutants of entomopathogenic fungi, 
, and 
, were screened from conidia irradiated with carbon ion beams at doses ranged from 100 to 1000 Gy. Sabouraud dextrose broth (SDB) and Sabouraud dextrose agar (SDA) were used for the screening. The wild-type strain of the three fungal species formed colonies with abnormal shape on SDA at 33
C. For 
, 41 isolates that could normally grow in SDB at 35C were successfully obtained, and finally 5 isolates were selected as potential mutants that will be used for further studies. Any normal colonies could not be obtained from SDA at 35C. The results show that ion beam irradiation is useful to generate thermotolerant mutants of entomopathogenic fungi. However, thermotolerant mutants of 
and 
have not been obtained yet.